Submitted for Publication: November 24, 2013; final revision received February 26, 2014; accepted March 2, 2014.
Published Online: April 17, 2014. doi:10.1001/jamaoto.2014.427.
Study concept and design: Nelson, Hinojosa.
Acquisition, analysis, or interpretation of data: Nelson, Hinojosa.
Drafting of the manuscript: Nelson, Hinojosa.
Critical revision of the manuscript for important intellectual content: Nelson, Hinojosa.
Obtained funding: Hinojosa.
Administrative, technical, and material support: Nelson, Hinojosa.
Study supervision: Nelson, Hinojosa.
Conflict of Interest Disclosures: None reported.
Funding/Support: Financial support for this project was provided by the Bloom Temporal Bone Laboratory Endowment at the University of Chicago and the Department of Otolaryngology Temporal Bone Histopathology Laboratory at the Wake Forest University School of Medicine.
Role of the Sponsor: The sponsors had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Additional Contributions: The authors acknowledge the contribution of Thomas Krausz, MD, Director of Anatomic Pathology at the University of Chicago, for the experimental design of this project; Adam Markaryan, PhD, at the House Ear Institute, for the development of immunostaining techniques; Vytas Bindokas, PhD, at the University of Chicago Microscopy Core Facility, for obtaining the photomicrographs presented in this article, and Robert I. Kohut, MD, at the Wake Forest University School of Medicine, for promoting the study of temporal bone proteoglycans. Dr Markaryan was a paid employee in our laboratory when the techniques were developed. The Core Facility charges our laboratory for use of its equipment and the expertise of Dr Bindokas.